ttbs buffer (Thermo Fisher)

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Thermo Fisher ttbs buffer
Ttbs Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ttbs+buffer/Tween+20/pm41097014-397-3-40
Average 99 stars, based on 1 article reviews

ttbs buffer - by Bioz Stars, 2026-10

99/100 stars

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Incubation:

Article Title: Pro-inflammatory cytokines activate hypoxia-inducible factor 3α via epigenetic changes in mesenchymal stromal/stem cells.
Article Snippet: .. After three washes for 10 min with TTBS buffer (50 mM Tris-HCl, pH 8.0, 150 mM NaCl, and 0.5% Tween-20), the membranes were incubated with horseradish peroxidase-conjugated anti-rabbit 9SCiEntifiC RepoRts | (2018) 8:5842 | DOI:10.1038/s41598-018-24221-5 antibody (1:10.000, Amersham, GE Healthcare, Life Technologies, Europe) for 1 h at room temperature and then washed for 15 min with TTBS buffer. .. The resulting immunoblots were detected using Amersham ECL Plus (GE Healthcare, Life Technologies, Europe).

Article Title: Mechanistic Insights into Cytokinin-Regulated Leaf Senescence in Barley: Genotype-Specific Responses in Physiology and Protein Stability
Article Snippet: Membranes were air-dried, blocked for 1 h at room temperature (RT) with 5% non-fat dry milk (Bio-Rad, Hercules, CA, USA), and incubated for 1 h at RT with gentle agitation, followed by overnight incubation at 4 °C with primary antibodies specific to: RCA (AS10 700; 1:5000, 5 μg of protein per lane), PsbO (AS06 142-33, 1:5000, 5 μg of protein per lane—ppl), PsbA (D1, AS05 084; 1:1000, 5 μg ppl), Lhcb1 (AS01 004; 1:1000, 15 μg ppl), Lhcb5 (CP26, AS01 009; 1:1000, 5 μg ppl), PsaB (AS10 695; 1:1000, 5 μg ppl), Lhca4 (AS01 008; 1:1000, 15 μg ppl), SAG12 (AS14 2771; 1:1000, 25 μg of protein per lane), and eEF1a (AS10 934; 1:5000, 5 μg of protein per lane, loading control) (all from Agrisera, Vännäs, Sweden). .. After washing with TTBS buffer (0.05% Tween 20, 20 mM Tris, 500 mM NaCl), membranes were incubated for 1 h at RT with horseradish peroxidase-conjugated secondary antibody (AS09 602; 1:5000–1:10000), followed by colorimetric detection using the PierceTM DAB Substrate Kit (Thermo Fisher Scientific, Waltham, MA, USA) for 5–10 min. .. Protein bands intensities were quantified via densitometry using ImageJ (v.1.52, NIH) [ ].

Article Title: Mechanistic Insights into Cytokinin-Regulated Leaf Senescence in Barley: Genotype-Specific Responses in Physiology and Protein Stability.
Article Snippet: Membranes were air-dried, blocked for 1 h at room temperature (RT) with 5% non-fat dry milk (Bio-Rad, Hercules, CA, USA), and incubated for 1 h at RT with gentle agitation, followed by overnight incubation at 4 ◦C with primary antibodies specific to: RCA (AS10 700; 1:5000, 5 μg of protein per lane), PsbO (AS06 142-33, 1:5000, 5 μg of protein per lane—ppl), PsbA (D1, AS05 084; 1:1000, 5 μg ppl), Lhcb1 (AS01 004; 1:1000, 15 μg ppl), Lhcb5 (CP26, AS01 009; 1:1000, 5 μg ppl), PsaB (AS10 695; 1:1000, 5 μg ppl), Lhca4 (AS01 008; 1:1000, 15 μg ppl), SAG12 (AS14 2771; 1:1000, 25 μg of protein per lane), and eEF1a (AS10 934; 1:5000, 5 μg of protein per lane, loading control) (all from Agrisera, Vännäs, Sweden). .. After washing with TTBS buffer (0.05% Tween 20, 20 mM Tris, 500 mM NaCl), membranes were incubated for 1 h at RT with horseradish peroxidase-conjugated secondary antibody (AS09 602; 1:5000–1:10000), followed by colorimetric detection using the PierceTM DAB Substrate Kit (Thermo Fisher Scientific, Waltham, MA, USA) for 5–10 min. ..

Article Title: Dual recognition of H3K4me3 and H3K27me3 by a plant histone reader SHL
Article Snippet: .. After washing three times with 1 ml TTBS buffer (10 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.05% Tween-20), the slide was incubated with 500 nM GST-tagged recombinant SHL in 1 ml binding buffer (50 mM HEPES, pH 7.5, 50 mM NaCl, 5% glycerol, 0.4% BSA, 2 mM DTT) at RT for 2 h. The slide was then washed with TTBS three times and incubated with an anti-GST antibody (Thermo Fisher, CAB4169, 1:3000 dilution) in TBS with 1% BSA for 1 h at RT. .. After washing with TTBS three times, the slide was incubated with an anti-rabbit IRDye 650 secondary antibody (LI-COR, P/N 926-65020, 1:5000 dilution) in 1 ml TBS buffer with 1% BSA for 1 h. The slide was washed with TTBS three times, dried by centrifugation, and imaged at dual wavelengths of 532 and 635 nm on Axon GenePix 4000B (Molecular Devices).

In Vitro:

Article Title:
Article Snippet: .. in vitro kinase assays BRAF kinase assay – Recombinantly expressed GST-MEK diluted in TTBS buffer (20 mM Tris pH 7.5, 150 mM NaCl, 0.05% TWEEN-20) to 50 μg/mL in a volume of 100 μL was bound to the wells of a 96-well glutathione coated plate (Pierce Biotechnology). ..

Kinase Assay:

other:

Article Title: Dendritic cell–intrinsic LKB1-AMPK/SIK signaling controls metabolic homeostasis by limiting the hepatic Th17 response during obesity
Article Snippet: GMDCs were washed in PBS, snap-frozen in liquid nitrogen, and lysed in ice-cold buffer containing: 50 mM Hepes (pH 7.6) (Sigma-Aldrich), 50 mM NaF (Sigma-Aldrich), 50 mM KCl (MilliporeSigma), 5 mM NaPPi (Sigma-Aldrich), 1 mM EDTA (Thermo Fisher Scientific), 1 mM EGTA (Sigma-Aldrich), 1 mM DTT (Promega), 5 mM β-glycerophosphate (Sigma Aldrich), 1 mM sodium vanadate (Sigma-Aldrich), 1% NP40 (Sigma-Aldrich), and protease inhibitors cocktail (Complete, Roche).

Article Title: Selective inhibition of stromal mechanosensing suppresses cardiac fibrosis.
Article Snippet: Matrix-derived biophysical cues are known to regulate the activation of fibroblasts and their subsequent transdifferentiation into myofibroblasts, but whether modulation of these signals can suppress fibrosis in intact tissues remains unclear, particularly in the cardiovascular system.. Here we demonstrate across multiple scales that inhibition of matrix mechanosensing in persistently activated cardiac fibroblasts potentiates—in concert with soluble regulators of the TGFβ pathway— a robust transcriptomic, morphological and metabolic shift towards quiescence.. By conducting a meta-analysis of public human and mouse single-cell sequencing datasets, we identify the focal-adhesion-associated tyrosine kinase SRC as a fibroblastenriched mechanosensor that can be targeted selectively in stromal cells to mimic the effects of matrix softening in vivo.

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ttbs buffer (Thermo Fisher)

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